Endoscopic H. Pylori Isolation in Bangladesh

Q: What is endoscopic H. pylori isolation?

Endoscopic H. pylori isolation is a medical procedure that uses an endoscope (a thin, flexible tube with a camera) to collect samples from the stomach lining. These samples are then tested to detect the presence of Helicobacter pylori (H. pylori), a bacteria linked to gastritis, ulcers, and even stomach cancer.

Q: How long does the procedure take?

The endoscopy itself takes 10–15 minutes, but you may need to stay for observation for a short time after the procedure.

Helicobacter pylori (H. pylori) is a bacterium that infects the stomach lining, leading to conditions such as gastritis, peptic ulcers, and an increased risk of gastric cancer. Endoscopic H. pylori isolation is a diagnostic procedure performed to confirm the presence of the bacteria in the stomach using an upper gastrointestinal (GI) endoscopy. This procedure allows direct visualization and biopsy sampling for further testing.

Successful and effective endoscopic H. pylori isolation requires an experienced and expert doctor. Dr. Salahuddin Mahmud is a pediatric gastroenterology and hepatology specialist in Dhaka. He offers less painful and modern techniques for H. pylori isolation.

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Endoscopic H. Pylori Isolation in Bangladesh

Step-by-step procedure for endoscopic H. pylori isolation

Dr. Salahuddin Mahmud offers an effective and comfortable procedural technique for endoscopic H. pylori isolation. Here we mentioned his procedure step-by-step. Explore for better understanding:

Pre-procedure preparation

Patient evaluation & preparation

Conduct a detailed medical history and physical examination.
Check for symptoms such as persistent gastritis, bloating, nausea, or peptic ulcer disease.
Discontinue proton pump inhibitors (PPIs), antibiotics, and bismuth-containing medications at least 2 weeks before the test, as they can affect H. pylori detection.
Ensure the patient is fasting for 6-8 hours before the procedure to allow clear visualization of the stomach lining.

Informed consent

Explain the procedure, benefits, and possible risks to the patient (or guardian in case of a child).
Obtain written informed consent.

Endoscopic procedure

Patient positioning & anesthesia

The patient is positioned in the left lateral decubitus position.
Local anesthesia is applied to the throat using a lidocaine spray to minimize discomfort.
Sedation may be administered if necessary to keep the patient relaxed.

Insertion of the Endoscope

A flexible upper GI endoscope is carefully inserted through the mouth into the esophagus, stomach, and duodenum.
The stomach lining is visually inspected for inflammation, ulcers, or other abnormalities.

Biopsy sample collection

Targeted Biopsy from Stomach

A sterile biopsy forceps is inserted through the endoscope’s working channel.
Multiple mucosal biopsies (usually from the antrum and corpus of the stomach) are taken, as H. pylori colonization is often patchy.
The biopsy samples are collected and preserved for further testing.

Laboratory testing for H. pylori isolation

The collected biopsy samples are tested using one or more of the following methods:

Rapid Urease Test (RUT)

The biopsy sample is placed in a urease test medium.
If H. pylori is present, it produces urease, which breaks down urea and changes the color of the medium.
Results are typically available within 30 minutes to a few hours.

Histopathological Examination

The biopsy is fixed in formalin and sent to the pathology lab.
The sample is stained using Giemsa or H&E (Hematoxylin & Eosin) stain to detect H. pylori under a microscope visually.

Bacterial Culture (Gold Standard for Isolation)

The biopsy is placed in a specialized transport medium and sent to a microbiology lab.
The sample is cultured on selective agar media (such as Columbia agar with antibiotics) under microaerophilic conditions (5% oxygen).
H. pylori colonies typically appear within 3-7 days.

Polymerase Chain Reaction (PCR) Testing

DNA is extracted from the biopsy and tested using PCR to detect H. pylori-specific genes.
This is a highly sensitive method that also helps detect antibiotic resistance.

Post-procedural Care & patient guidance

Dr. Salahuddin Mahmud is very sincere and focused on post-procedural patient care and guidance. He is famous for his good behavior & skills. Here we mentioned his post-procedural care step-by-step:

The patient is monitored for 30-60 minutes post-procedure for any side effects from sedation.
Mild throat discomfort or bloating may occur but usually resolves quickly.
The patient is advised to resume normal eating and activities once fully awake.
If a biopsy results in a confirmed H. pylori infection, a treatment plan is prescribed, including triple or quadruple therapy with antibiotics and acid-suppressing medications.

Follow-Up

A follow-up test, such as a stool antigen or urea breath test, might be recommended 4-6 weeks after finishing treatment to confirm eradication.
Patients with ulcers or chronic gastritis may need additional endoscopic evaluations.

Why choose Dr. Salahuddin Mahmud for endoscopic H. pylori isolation?

Dr. Salahuddin Mahmud is the trusted and expert doctor in the field of H. pylori isolation. His qualifications, modern procedural technique, and experience are the main factors in choosing him. Here we mentioned his exceptional qualities; explore now: